The Biological Mass Spectrometry facility provides specialized MS services to the University of Michigan research community. The two major areas of focus of the lab are: 1) analysis of intact proteins, multi-protein complexes, and protein structure; and 2) identification and purification of compounds from complex mixtures coupled with quantitative analysis. Users can be trained on the instruments for walk-up use or samples can be submitted for analysis. The facility will move into approximately 1000 sq. ft. of newly renovated laboratory space in the spring of 2021.
Joseph Caruso, Manager
Chemistry Rm A525,
930 N. University Ave., Ann Arbor, MI, 48109
Cyclic IMS Q-TOF (Waters Corporation) In this state-of-the-art mass spectrometer, the traditional linear ion mobility region is replaced with a compact cyclic ion guide, leading to greater ion mobility resolution. This configuration introduces the unique ability to perform ion mobility/ion mobility and IMSn experiments. This system is supported by an M-Class ACQUITY UHPLC liquid chromatograph.
Q Exactive Ultra High Mass Range (UHMR) Orbitrap (Thermo Fisher Scientific) This MS offers high-resolution native intact mass identification (up to 80,000 m/z) and MS/MS capabilities for detailed analysis of protein complex stoichiometries, drug-associated assemblies, and top- and middle-down proteomic analyses.
TSQ Quantis Triple-Stage Quadrupole (Thermo Fisher Scientific) This system is optimized for targeted quantitation of a wide array of molecular species, including those found in complex mixtures, using single reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes of operation. This MS is supported by a Vanquish UHPLC liquid chromatograph.
AutoPurification Preparative Scale LC/MS (Waters Corporation) This system enables high-throughput separation and fractionation of compounds from naturally-occurring sources or from multi-step chemical synthesis strategies. Features of this platform include a SQD2 quadrupole mass spectrometer, photodiode array detector, and AutoPurify to automatically screen samples during processing.