Skip to Content

Search: {{$root.lsaSearchQuery.q}}, Page {{$}}

Biophysics Seminar Series

Dr. Ido Golding (University of Illinois Urbana-Champaign)
Friday, October 30, 2020
12:00-1:00 PM
Join us on Zoom:

The Biophysics Virtual Seminar Series presents:

Dr. Ido Golding - Professor of Physics, School of Molecular & Cellular Biology, University of Illinois Urbana-Champaign

"Illuminating bacterial individuality"

ABSTRACT: Single-cell measurements of mRNA copy numbers inform our understanding of stochastic gene expression, but these measurements coarse-grain over the individual copies of the gene, where transcription and its regulation take place stochastically. We recently combined single-molecule quantification of mRNA and gene loci to measure the transcriptional activity of an endogenous gene in
individual Escherichia coli bacteria. When interpreted using a theoretical model for mRNA dynamics, the single-cell data allowed us to obtain the probabilistic rates of promoter switching, transcription initiation and elongation, mRNA release and degradation. Unexpectedly, we found that gene activity can be strongly coupled to the transcriptional state of another copy of the same gene present in the cell, and to the event of gene replication during the bacterial cell cycle. These gene-copy and cell-cycle correlations demonstrate the limits of mapping whole-cell mRNA numbers to the underlying stochastic gene activity and highlight the contribution of previously hidden variables to the observed population heterogeneity.
Building: Off Campus Location
Location: Virtual
Event Link:
Event Password: Biophysics
Event Type: Livestream / Virtual
Tags: Biophysics, Free, Research, seminar, Virtual
Source: Happening @ Michigan from LSA Biophysics