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Applied Physics Seminar: Single-molecule microscopy and spectroscopy of plasmon-coupled fluorescence

Julie S. Biteen, Associate Professor of Chemistry and Associate Professor of Biophysics, College of Literature, Science, and the Arts, University of Michigan
Wednesday, January 22, 2020
12:00-1:00 PM
335 West Hall Map
Single-molecule microscopy and spectroscopy probe fluorescence emission in a direct, sensitive, and precise fashion. These measurements therefore enable us to observe and understand the strong light/matter interactions that occur when a dye molecule couples to the local surface plasmon resonance modes of a proximal metal nanoparticle. We have demonstrated improved brightness and photostability of dye molecules near gold nano-antennas, and we have extended this biocompatible fluorescence enhancement to fluorescent proteins in living cells. Furthermore, we have discovered that dye/nanoparticle coupling leads to a predictable mislocalization of the emission position and we are understanding the shifts in localization, polarization, and spectrum that arise in plasmon-coupled fluorescence. Overall, we are creating a flexible technology for super-resolution, real-time imaging.
Building: West Hall
Event Type: Lecture / Discussion
Tags: Physics
Source: Happening @ Michigan from Applied Physics