Applied Physics Seminar: Single-molecule microscopy and spectroscopy of plasmon-coupled fluorescence
Julie S. Biteen, Associate Professor of Chemistry and Associate Professor of Biophysics, College of Literature, Science, and the Arts, University of Michigan
Wednesday, January 22, 2020
335 West Hall Map
Single-molecule microscopy and spectroscopy probe fluorescence emission in a direct, sensitive, and precise fashion. These measurements therefore enable us to observe and understand the strong light/matter interactions that occur when a dye molecule couples to the local surface plasmon resonance modes of a proximal metal nanoparticle. We have demonstrated improved brightness and photostability of dye molecules near gold nano-antennas, and we have extended this biocompatible fluorescence enhancement to fluorescent proteins in living cells. Furthermore, we have discovered that dye/nanoparticle coupling leads to a predictable mislocalization of the emission position and we are understanding the shifts in localization, polarization, and spectrum that arise in plasmon-coupled fluorescence. Overall, we are creating a flexible technology for super-resolution, real-time imaging.
|Event Type:||Lecture / Discussion|
|Source:||Happening @ Michigan from Applied Physics|