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Research Ethics & Strategy What's
the picture at right? Our laboratory exploits the microstructure of natural behavior to discover new truths about brain and psychology, instinctive behavior and affective reactions. Natural affective reactions and instinctive behaviors provide windows into controls of sensory pleasure, fear, or executive action syntax functions. Combined with conventional behavioral neuroscience techniques (instrumental & classical conditioning) and neuroscience techniques for manipulating brain function (painless drug microinjections), we explore how brain systems generate psychological processes. |
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Research Techniques Neuroscience manipulations - We use several neuroscience techniques that selectively and painlessly alter a single neural system in the brain. Laser activation & inhibition of targeted brain systems. We use optogenetics techniques to painlessly manipulate the brain with light (photo below). A blue laser light delivered via optic fiber to the brain selectively activates a virus-targeted neural system. Specificity is obtained by previously microinjecting a tiny quantity of virus containing RNA instructions for builidng photoreceptor molecules into a brain structure under anesthesia and laser probes are implanted in the brain to selectively activate the cells expressing the virus (adjacent photo depicting a recreation of laser stimulation of the ventral striatum by either a blue (473nm) or red (635nm) laser). The virus selectively causes a chosen set of neurons to become light sensitive, and open ion channels to stimulate the neurons when the laser is on. Conversely, a differently colored red or other laser light inhibits the neurons via different photoreceptors. These techniques can painlessly control 'wanting' and 'liking' or other emotional processes. The green photo below shows neurons in the basolateral amygdala (left) and nucleus accumbens (right) making a green fluorescent protein after being infected by virus. Drug microinjections into brain. To pinpoint sites of brain neurochemical circuits controlling emotion we developed a Fos plume mapping technique to localize function using painless brain microinjections that place drugs into a selected brain
region. We also sometimes histological techniques such as Fos
plume maps s. ![]() Ethical testing - We strive for good research ethics in affective neuroscience. Our research uses painless behavioral testing procedures for all our animal subjects, and never involves significant suffering. All neuroscience manipulations (used only for rat and mouse studies) are conducted painlessly or under appropriate anesthesia. Our behavioral tests of reward and pleasure are not painful. Whenever our research collaborations involve human subjects the studies are carried out by experts who specialize in those studies. All research techniques used in the laboratory have been approved by the appropriate ethical review boards within the university and by US federal grant agencies (National Institutes of Health & National Science Foundation). |
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