Skip to Content

Search: {{$root.lsaSearchQuery.q}}, Page {{$root.page}}

PHYSICS GRADUATE STUDENT SYMPOSIUM<br>Measuring the Stark Shift in the Two-Photon Cross Section of Fluorescent Proteins

  1. All News & Features
  2. All Events
    1. Archived Events
      1. 2013
      2. 2012
      3. 2011
      4. 2010
      5. 2009
      6. 2008
      7. 2007
      8. 2006
      9. 2005
      10. 2003
      11. 2002
      12. 2001
      13. 2000
      14. 1999
      15. HEP Astro
      16. Astronomy Colloquium
      17. Biophysics Seminar
      18. CM - AMO Seminars
      19. CM Theory Seminars
      20. Complex Systems
      21. Department Colloquia
      22. Quantitative Biology Seminars
      23. HET Brown Bag Series
      24. HET Seminars
      25. Life After Grad School Seminars
      26. Farrand Memorial Lecture
      27. Workshops & Conferences
        1. <b>FOUNDATION OF MODERN PHYSICS WORKSHOP</b><br>Foundations of Ordinary Quantum Mechanics Workshop
        2. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM</b><br>Radiations, Photons and Interference
        3. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM</b><br>Ultra-Thin, Smooth and Low Loss Al-Doped Ag Film and Its Application in Optoelectronic Devices
        4. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM</b><br>Hyperbolic Metamaterials Based on Graphene-Dielectric Multilayers
        5. PHYSICS GRADUATE STUDENT SYMPOSIUM<br>Measuring the Stark Shift in the Two-Photon Cross Section of Fluorescent Proteins
        6. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM</b><br>Parton Dynamics in High Energy Proton Proton Collisions at PHENIX<br>
        7. PHYSICS GRADUATE STUDENT SYMPOSIUM<br>Information Divergence Estimation in Signal Processing and Machine Learning
        8. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM<b><br>Galaxy Evolution in X-Ray Selected Clusters and Groups in Dark Energy Survey Data
        9. <b>Physics Graduate Student Symposium</b><br>Engineering the Properties and Defects of InAsSb by Atomic Surface Structure
        10. <b>PHYSICS GRADUATE STUDENT SYMPOSIUM</b><br>Spintronic Devices and Spin Physics in Bulk Semiconductors
        11. MCTP Workshop
      28. Miscellaneous
      29. Saturday Morning Physics
      30. Special Lectures
      31. Search Events
  3. Special Lectures
  4. K-12 Programs
  5. Saturday Morning Physics
  6. Seminars & Colloquia
Wednesday, July 15, 2015
12:00 AM
340 West Hall

Two-photon fluorescence imaging has vast improvements over its one-photon counter part for imaging biological systems, including reduced contributions due to scattering, z-axis dependent focusing, ease of detection geometry and reduced out of focus photobleaching.  Central to the development and versatility of fluorescence imaging is green fluorescent protein (GFP) and its ability to be expressed in various mammalian systems without the need for species-specific cofactors.  Manipulation of GFP’s chromophore amino acid structure has also enabled the creation of many GFP mutants with a range of emissions wavelengths spanning from the blue to the red.  Stark spectroscopy has revealed that GFP mutant emission wavelengths are dependent on the local electric field produced by the chromophore’s amino acid sequence, suggesting a dependence on the chromophore’s difference dipole moment. Coincidentally, two-photon absorption is also dependent on the difference dipole moment, suggesting that changing the electric field environment of the protein can shift the emissions spectrum of the protein’s two-photon response.  If it is possible to characterize and monitor the shift in two-photon emissions from an applied electric field, this effect offers many potential improvements to two-photon fluorescence imaging of biological processes, like neuron action potential events, which involve the movement of ions across the neuronal membrane.

Lunch will be served in the Don Meyer Common Room at 11:50 a.m.

Speaker:
Elizabeth Maret (U-M Physics Graduate Student)