Please note that you may be required to obtain permission or training from the equipment caretaker before use.
The MCDB Imaging Labs contain advanced microscope equipment, image capture systems, software, and other resources available to all MCDB researchers and collaborators. This shared equipment is provided by the department to best aid as many research labs as possible.
Directions to the Imaging Suite
Take the east elevator. Scan your badge to select B to go down to the basement. Turn left out of the elevator and scan badge to access the microscope suite.
The MCDB department provides several research-grade microscopes with image capture systems to assist researchers with their sample imaging. [Room location in brackets.]
Leica SP8 laser scanning confocal microscope with 405nm and white light (470-670nm) laser systems, gating, spectral detection, resonant/tandem scanner, and STED super resolution imaging with 592, 660, and 775nm depletion lines. [B130]
Leica SP5 laser scanning confocal microscope with 405, 458, 476, 488, 514, 561, and 633nm laser excitation lines available, spectral detection, superfast resonant scanner, and many other features. [B134]
Andor/Olympus spinning disk confocal microscope imaging system with 405, 458, 488, 514, 561, and 640nm laser excitation lines, 10x, 20x, 60x, and 100x objective lenses, a FRAP module, continuous focus feature, streaming mode, Photometrics dual view DV2 module, live cell incubation chamber, lens heater, and an Andor iXon Ultra CCD camera. [B122]
Olympus IX81 inverted fluorescence microscope with motorized Z movement, a Retiga 1300R Fast, cooled, monochrome cameraphase contrast, and 4x, 10x, 20x, and 40x objective lenses. [B138]
Leica MZ 16F fluorescence stereomicroscope with trans illumination base, 1.4MP and 5MP, color cameras available, and microscope dichroics for UV, green (GFP), and red (RFP) fluorescence detection. [B138]
Nikon E800 fluorescence microscope with phase contrast, DIC, UV, green (GFP), and red (RFP) LED excitation, a Nikon DS-Fi3 5.9MP color digital camera, and 4x, 10x, 20x, 40x, and 100x oil objective lenses. Provides color imaging with fluorescence sensitivity. [B138]
Zeiss Axio Imager Z1 fluorescence microscope with Apotome, DIC contrast, UV, green (GFP), and red (RFP) LED excitation, a Zeiss Axiocam MrM camera, and 2.5x, 10x, 20x, 40x oil, 63x oil, and 100x oil objective lenses. Automated z-stage. [B138]
Jeol JEM 1400plus Transmission Electron Microscope [B118]
Microscope Imaging Suite Specimen Preparation lab in B144A.
Leica 3040S cryostat sectioning, paraffin sectioning microtome station, Richiert Ultracut E ultra microtomes, paraffin and resin heating ovens.
If you need special specimen storage for use on the microscopes, please arrange with Gregg.
Molecular Core: Access from the 3C LER.
Room 3215: StepOne Plus qPCR, 7500 Fast qPCR, CFX Connect qPCR, Nanodrop Lite, Qubit 2.0 fluorometer, Pipette Puller and evaluation microscope.
Room 3235: Tecan M200 plate reader, Tecan F200 plate reader, LiCor CLx IR imager, LiCor Fc UV and luminescence gel imager, GE Typhoon phosphor imager, Lyophilizer.
Please speak with Gregg Sobocinski (firstname.lastname@example.org or 734.615.2034) before using any of these microscopes. If you have any questions about microscope technologies or accessories available within the MCDB department, please feel free to speak with Gregg. He will be happy to assist you with using these microscopes to their optimal capabilities, or will recommend other University of Michigan resources, as appropriate.
To reserve confocal or electron microscope equipment please use the Confocal and EM Sign-up Calendars (link below). Access is granted after training is complete. An active shortcode is required.