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BIO 174 Recombinant DNA

Recombinant DNA

Intravenous immunoglobulin (IgG) therapy can be used to treat a variety of medical conditions, including immune deficiencies, acute infections, and autoimmune disorders. Traditionally, these products are manufactured from human blood and plasma, but this is very expensive and labor-intensive. Currently, a four dose course of traditionally produced IgG therapy for a 70 kg person at 2 g/kg would cost $25,000-$26,000 (Jordan et al).

You are working for a biotech company that is beginning to conduct research to develop a recombinant DNA technology so that cultured bacteria can be engineered to produce IgG. Write a memo to your funders justifying why you have decided to reallocate resources to pursue recombinant DNA technologies in lieu of traditional manufacturing processes.

In your memo, be sure to explain:

1. How the process would work: What steps would be involved in creating recombinant bacteria to produce IgG? What reagents or products would be needed at each step? How you would obtain them?

2. Why the process would be less expensive than traditional methods: Why is bacterial production of IgG more efficient than human production?

Items to keep in mind:

  • External references are not required, but if they are used they should be cited using MLA format.
  • You should take care to carefully edit and proofread your entry.
  • The entry should be 400 to 500 words long.

Jordan, S. C.; A. A. Vo; A. Peng; M. Toyoda; D. Tyan. “Intravenous Gammaglobulin (IVIG): A Novel Approach to Improve Transplant Rates and Outcomes in Highly HLA-Sensitized Patients.” American Journal of Transplantation. 2006; 6(3): 459-466.

 

Peer Review Guidelines:

  • Print and read over your peer’s essay to quickly get an overview of the piece.
  • Read the essay more slowly keeping the rubric in mind.
  • Highlight the pieces of texts that let you directly address the rubric prompts in your online responses.
  • In your online responses, focus on larger issues (higher order concerns) of content and argument rather than lower order concerns like grammar and spelling.
  • Be very specific in your responses, referring to your peer’s actual language, mentioning terms and concepts that are either present or missing, and following the directions in the rubric.
  • Use respectful language whether you are suggesting improvements to or praising your peer.

Rubric Prompts:

1. Would this entry be understandable to a person with minimal scientific background?  Which parts were difficult to understand? Which parts would be easy to understand?
2. Does the memo provide a clear description of the process of creating recombinant bacteria? Does it describe the reagents and products involved in the process?
3. How does the memo define or explain the key concepts involved in the process?
        a. Template DNA
        b. Vector
        c. Plasmid
        d. Restriction enzyme
        e. DNA ligase
        f. Transformation
4. How does the memo compare bacterial production of IgG and human production?

Revision Prompt

Revising writing means re-seeing it, and the process of reading and commenting on the writing of others as well as receiving feedback from your peers gives you a way of seeing your own writing differently. Meaningful revision means changes at the sentence and paragraph level, usually involving a minimum of three sentences. You will not receive full credit for revision unless you make meaningful revisions to your writing.

Revision Guidelines:

  • Re-read the prompt
  • Re-read the rubric and consider what a complete and effective response would include, noting what you do not fully address
  • Make a list of effective content you noticed in the writing of your peers
  • Read and summarize the feedback you received from your peers
  • With these things in mind, re-read your draft and mark places where you can improve the content
  • Revise and submit your response
  • After completing this assignment, please follow the link to respond to a survey about the writing assignment:

Checklist from Recombinant DNA Peer Review Rubric:

1. The memo should be understandable to a person with minimal scientific background.
2. The memo should provide a clear description of the process of creating recombinant bacteria as well as the reagents and products that are involved.     
3. The memo should define or explain the following key concepts involved in the process of creating recombinant bacteria.
        a. Template DNA
        b. Vector
        c. Plasmid
        d. Restriction enzyme
        e. DNA ligase
        f.  Transformation
4. The memo should compare bacterial production of IgG and human production.