Applied Physics Seminar: Mechanobiology of Membrane: from Mechanosensitive Channels to Artificial Cells
Allen Po-Chih Liu, Associate Professor of Mechanical Engineering, College of Engineering, Associate Professor of Biomedical Engineering, College of Engineering and Medical School and Associate Professor of Biophysics, College of Literature, Science, and t
Abstract:
Biological membranes are involved in a large number of cellular processes including cell migration, membrane trafficking, and cell signaling. Significant amount of work have elucidated the molecular machineries that regulate dynamic membrane-based processes. In parallel, there are growing interests in recent years in trying to understand how mechanical state of the cells are utilized as a regulatory input to control cellular processes. My lab is interested in studying the mechanochemical responses of biological systems. In this talk, I will present two projects related to this theme. On the cellular level, we have reconstituted the function of a bacterial mechanosensitive channel MscL in mammalian cells. Using this system, we investigated the role of actin cytoskeleton in mediating local membrane tension that activates MscL, and whether MscL expression might influence 3D confined migration, during which cells experience significant stress. On the synthetic level, we are building artificial systems that can sense mechanical input and transduce a biochemical response. To this end, we are attempting to build artificial platelets that mimic the functionalities of natural platelets. I will discuss several modular platforms that we have developed that together will integrate into functional artificial cells. Together, our work will provide basic understanding of cellular mechanotransduction and potential applications of force-activated synthetic biology.
Biological membranes are involved in a large number of cellular processes including cell migration, membrane trafficking, and cell signaling. Significant amount of work have elucidated the molecular machineries that regulate dynamic membrane-based processes. In parallel, there are growing interests in recent years in trying to understand how mechanical state of the cells are utilized as a regulatory input to control cellular processes. My lab is interested in studying the mechanochemical responses of biological systems. In this talk, I will present two projects related to this theme. On the cellular level, we have reconstituted the function of a bacterial mechanosensitive channel MscL in mammalian cells. Using this system, we investigated the role of actin cytoskeleton in mediating local membrane tension that activates MscL, and whether MscL expression might influence 3D confined migration, during which cells experience significant stress. On the synthetic level, we are building artificial systems that can sense mechanical input and transduce a biochemical response. To this end, we are attempting to build artificial platelets that mimic the functionalities of natural platelets. I will discuss several modular platforms that we have developed that together will integrate into functional artificial cells. Together, our work will provide basic understanding of cellular mechanotransduction and potential applications of force-activated synthetic biology.
| Building: | West Hall |
|---|---|
| Event Type: | Lecture / Discussion |
| Tags: | Biomedical Engineering, Physics |
| Source: | Happening @ Michigan from Applied Physics |
